VIII WORKSHOP

RAPID METHODS AND AUTOMATION

IN FOOD MICROBIOLOGY

 

Key-note speaker and Major lecturer

Professor Dr. Daniel Y. C. Fung

(Kansas State University, Manhattan, Kansas, USA)

 

Universitat Autònoma de Barcelona

Facultat de Veterinària

Lecture hall

(with sessions in laboratory)

 

Bellaterra (Cerdanyola del Vallès), Barcelona

24th to 27th November 2009

 

 Directors

Marta Capellas Puig and Josep Yuste Puigvert

 

Organized by

Centre Especial de Recerca Planta de Tecnologia dels Aliments

Departament de Ciència animal i dels aliments

 

 

 

 

 


PURPOSE

 

To broaden and spread the theoretical and practical knowledges about innovative methods for rapid detection, count, isolation and characterization of foodborne and waterborne microorganisms.

 

 

KEYWORDS

 

Rapid microbiology · Automation · Miniaturization · Food safety · Pathogens · Food analysis

 

 

DESIGNED FOR

 

Directors and technicians of laboratories, consultancies and industries of food, and other sectors (clinical, pharmaceutical, cosmetic, chemical, environmental, and so on); food inspectors and other government staff; people from microbiology companies; university teachers, technical staff, and graduate and postgraduate students; people from other research centres; and so on.

 

 

PROGRAMME (REGISTRATION IS STILL OPENED, BUT NOT FOR LABORATORY SESSIONS, AND DEMONSTRATIONS ON FRIDAY, 27th NOVEMBER)

 

Tuesday, 24th November

 

8:30-9:30 a.m. Handout material delivery and welcome

9:30-10:15 a.m. Opening lecture by Dr. Cécile Lahellec (Agence Française de Sécurité Sanitaire des Aliments –AFSSA–, Alfort, France):

·        International cooperation in food microbiology

10:15-11:15 a.m. Dr. Daniel Y. C. Fung:

·        Sampling and sample preparation of solid and liquid foods, surfaces, and air

·        Miniaturization: microtiter plate, most probable number (MPN), viable cell counts, and so on. Diagnostic kits

11:15-11:45 a.m. Break

11:45 a.m.-12:45 p.m. Dr. D. Y. C. Fung:

·        Methods for viable cell counts: hydrophobic grid, spiral plating, flow cytometry, direct epifluorescent filter technique (DEFT), and so on

·        Other methods to detect microorganisms: Fung’s double tube, Lee tube, Fung-Yu tube, and so on.

12:45-2:15 p.m. Lunch

2:15-3 p.m. Dr. D. Y. C. Fung:

·        Methods for viable cell counts, based on electric impedance, conductance and capacitance

·        Methods for viable cell counts, based on bioluminiscence (ATP analysis) and colorimetry

3-4 p.m. Dr. Armand Sánchez Bonastre (Universitat Autònoma de Barcelona –UAB–, Bellaterra –Cerdanyola del Vallès, Barcelona–):

·        “La polymerase chain reaction (PCR)” [“Polymerase chain reaction (PCR)”]

4-4:30 p.m. Sr. Martín Iorlano (APSALAB, SL, Reus, Tarragona):

·        “Puesta a punto e implantación de la PCR para Salmonella spp. en piensos y materias primas” [“Implementation of PCR for Salmonella spp. in feed and raw materials”]

4:30-4:40 p.m. Break

4:40-6:50 p.m. Microbiology companies: 2 exhibitions:

* The industrial traditional microbiology: first dilution; homogenization; spiral dilution; colony counting; and database communication among all steps (IUL, SA)

* BAX Q7 system: simple and consistent PCR, designed for the needs of food industry, and reference and official control laboratories (Oxoid, SA –part of Thermo Fisher Scientific, Inc–)+

3 multimedia:

* BD DS: systems and products for water and food microbiological analyses, and environmental control (Becton Dickinson, SA)

* Application of real-time PCR to routine detection of food pathogens (GeneSystems, SA –part of Pall Life Sciences–)

* Development and validation of Brucella spp. molecular typification assays by conventional PCR and real-time PCR (Roche Diagnostics, SL)

6:50 p.m. Merienda

 

Wednesday, 25th November

 

8:30-11 a.m. 1st laboratory session (mornings group) FULL!

11-11:15 a.m. Break

11:15 a.m.-1 p.m. Dr. D. Y. C. Fung:

·        Immunological methods for microbial and toxin identification: immunomagnetic separation, ELISA and ELFA, reversed passive latex agglutination, lateral immunomigration, immunoprecipitation, and so on

1-2:15 p.m. Lunch

2:15-3 p.m. Dr. Teresa Esteve Nuez (Centre de Recerca en Agrigenòmica –CRAG–, CSIC-IRTA-UAB consortium, Barcelona):

·        Organismos modificados genéticamente (OMGs): detección, legislación y evaluación de riesgos” [“Genetically modified organisms (GMOs): detection, legislation and risk assessment”]

3-3:45 p.m. Mr. David Tomás Fornés (ainia.centro tecnológico, Paterna, Valencia):

·        Control de calidad interno en laboratorios de microbiología” [“Internal quality control in microbiology laboratories”]

3:45-4 p.m. Break

4-6:30 p.m. Microbiology companies: 4 exhibitions:

* Microbiology laboratory automation and traceability. Rapid microbiology solutions (AES CHEMUNEX España, SA)

* Microbiology laboratory automation for detection and counting: a fact (bioMérieux España, SA)

* ATP – Bioluminiscence and concentration of disinfectants for HACCP audits. Immunomagnetic separation for foodborne pathogen detection (MicroPlanet Laboratorios, SL: BioControl Systems, Inc / LIOFILCHEM, srl)+

* AmpliGrid technology for PCR, real-time PCR and sequentiation. Also from one single cell! (Olympus España, SAU)

6:30 p.m. Merienda

 

Thursday, 26th November

 

8:30-11 a.m. 2nd laboratory session (mornings group) FULL!

11-11:15 a.m. Break

11:15-12 a.m. Dr. D. Y. C. Fung:

·        Genetic methods for microbial identification: hybridization (DNA and RNAr probes); polymerase chain reaction (PCR); DNA characterization (fingerprinting, riboprinting); biosensors, biochips and microchips; proteomics; and so on

12 a.m.-12:45 p.m. Dr. Ferran Ribas (Comisión de Normalización y Validación CNV–, SEM, Madrid):

·        Estudios de equivalencia europeos entre métodos para enumerar Escherichia coli y enterococos en aguas de baño” [“European equivalence studies between methods for Escherichia coli and enterococci enumeration in recreational waters”]

12:45-1 p.m. Official picture

1-2:15 p.m. Lunch

2:15-3:15 p.m. Open discussion about instrumentation in food microbiology, world trade trends, and other current topics. Conclusions. Participants:

-         Chairman: Dr. José Juan Rodríguez Jerez (Universitat Autònoma de Barcelona)

-         Dr. Daniel. Y. C. Fung

-         Dr. Cécile Lahellec

-         Dr. Ferran Ribas Soler

-         Mr. Xavier Lizana Alcazo (ACONSA Asesoría y Consultoría Sanitaria, SL)

-         Mr. Francesc García Bejarano (bioMérieux España, SA)

-         Mr. Juan Antonio García González (Applied Biosystems)

3:15-4 p.m. Official adjournment of the workshop: attendance-certificates-giving and celebration

4-6:30 p.m. Microbiology companies: 4 exhibitions:

* Rapid methods related to quality management in the food industry (3M España, SA)

* Implementation of foodborne pathogen detection systems at quality control laboratories (Applied Biosystems)

* Optimization at quality laboratory: pathogen analysis and surface hygiene validation (Bioser, SA)

* Eppendorf: a complete system for real-time PCR (Eppendorf)

 

Friday, 27th November

 

9-11 a.m. 3rd laboratory session (mornings group) FULL!

11:15 a.m.-12:15 p.m. Demonstration on Use of internet for applications of predictive microbiology (limited attendance), by Ms. Montse Vila Brugalla (Barcelona Public Health Agency) FULL!

12:15-1 p.m. Demonstration on Electrochemical immunosensors for pathogen detection (limited attendance), by Drs. Mª Isabel Pividori Gurgo and Anabel Lermo Soria, and Ms. Susana Liébana Girona (UAB) FULL!

 

 

LABORATORY SESSIONS FULL!

 

In these sessions, which will be given by Drs. Mª Manuela Hernández Herrero (manuela.hernandez@uab.cat) and Artur Xavier Roig Sagués (arturxavier.roig@uab.cat), different rapid methods will be assayed: Surface and air samplers: contact plates, Rodac plates, contact slides, Sampl’air, MicroBio. Homogenizer Pulsifier; blender Smasher. Dilumat S and Dilubag. Dilucup/Dilushaker. Chromogenic culture media: ASAP, SMS, ESIA, REBECCA, ALOA, BACARA, Baird-Parker RPF agar, chromID Coli, chromID Salmonella, chromID O157:H7, chromID Ottaviani Agosti, Brilliance Salmonella agar (Oxoid Salmonella Precis method), Brilliance Listeria agar (Oxoid Listeria Precis method), Rose-Gal BCIG agar, SESAME Salmonella test, COMPASS Salmonella agar, COMPASS Listeria agar, CONFIRM’ L. mono agar, COMPASS Bacillus cereus agar, BBL CHROMagar Salmonella, BBL CHROMagar O157, BBL CHROMagar Listeria, BBL CHROMagar Staph aureus, Chromatic DETECTION Salmonella, Compact Dry Plate ETB, Compact Dry Plate SL, Compact Dry Plate X-SA. Rotating plater Twister. Spiral plater WASP II. Colony counter EC2. Petrifilm (plates and reader). NEO-GRID. Easygel. SimPlate. TEMPO (miniaturized and automated MPN). Diagnostic kits and readers: API, BBL Enterotube II, BBL Crystal ID, RapID ONE, O·B·I·S·, Microbact, Microgen ID, EnteroPluri-Test, FOOD-SYSTEM. ATP – Bioluminiscence: luminometers Luminomat, Clean-Trace NG, LIGHTNING MVP. Colorimetry: Clean Test, drink water kit P/A, KIT PRO. Immunology: ELFA/ELISA (VIDAS UP E. coli O157:H7: phage recombinant protein technology, Alert for histamine), reversed passive latex agglutination (Microgen latex, Oxoid latex test), lateral immunomigration (RapidChek, BioKits RAPID 3D –allergens–, Reveal –pathogens, allergens–, VIP), immunoprecipitation (1-2 Test for Salmonella). Others. Explanation about hands-free "Pop-up" adhesive tape sampling, Easygel, Fung’s double tube and Fung-Yu tube.

Attendance will be limited to 25 people per session.

 

 

MICROBIOLOGy companies: EXHIBItion

 

People from companies will explain and show how their equipments and products work: Samplers: air IDEAL, Spin Air, Spin Air Slave, Spin Air Basic, Basic Air. Pinch dilutor. Preparator of culture media, and automated pourer stacker APS One. Plate Handler. Homogenizers: Masticator Silver Panoramic, Masticator Compact Panoramic; blender Smasher. Dilumat S and Dilubag. Dilutors: Smart Dilutor, Bio Dilutor. Chromogenic culture media: SESAME Salmonella test, COMPASS Listeria agar, CONFIRM’ L. mono agar. Spiral plater Eddy Jet. Colony counters and inhibition halo readers: Manual Colony Counter, Haloes-Caliper, Flash & Go. Petrifilm. Staining automation: Poly Stainer. Real-time microbiology CHEMUNEX: BactiFlow. TEMPO (miniaturized and automated MPN). BiOLOG identification system. eviSENSE monitoring system. ATP – Bioluminiscence: luminometer LIGHTNING MVP. Colorimetry: Path-Check Hygiene. Immunology: ELFA (VIDAS), lateral immunomigration (BioKits RAPID 3D –allergens– + multichannel Ovation pipette), immunomagnetic separation PickPen. Molecular biology: PCR (7300, 7500, 7500 Fast, 7900, Step One, Step One Plus; BAX Q7; ADIAFOOD; LightCycler; AmpliGrid; Mastercycler ep realplex; Assurance GDS; GeneDisc; kits Salmofast, Listerfast, Legiofast, Campylofast, Tyrofast, Sakafast). AirFree. Others.

 

 

PROFILES OF LECTURERS AND OTHER PARTICIPANTS

 

Dr. Daniel Y. C. Fung (dfung@ksu.edu) is a professor of food science in the Department of Animal sciences and industry at Kansas State University (KSU), in Manhattan, Kansas (USA). His speciality is food microbiology, and he has gained an international reputation as a lecturer on rapid and miniaturized methods and automation. He has more than 800 publications, including papers in scientific journals, books and presentations in congresses. Highly Qualified Visiting Professor since 2003, mention given by the International Graduate School of Catalonia (Departament d’Universitats, Recerca i Societat de la Informació); and Distinguished Professor of Veterinary College at UAB since 2006. Director of the annual international workshop on Rapid methods and automation in microbiology, which is held in Manhattan, KS and has reached the 29th edition. Recipient of the 1997 International Award given by the Institute of Food Technologists (IFT), because of the uniqueness of this workshop series; the 2001 Waksman Outstanding Educator Award given by the Society for Industrial Microbiology; the 2005 KSU College of Agriculture Excellence in Graduate Teaching Award; the 2006 IFT Carl R. Fellers Award, for a distinguished career in Food science and technology; and the 2007 Inaugural Outstanding Educator in Food Safety Award given by Food Safety magazine and ConAgra Foods, Inc., for his teaching career with more than 18,000 students and 112 graduate students (33 Ph.D. and 79 M.S.) completed as the major professor. Senior associate editor of Journal of Rapid Methods and Automation in Microbiology. Fellow of the American Academy of Microbiology, the IFT and the International Academy of Food Science and Technology. He presented an invited lecture at Pasteur Institute in Paris (France) during the 100th year commemoration of Pasteur’s death in 1995.

 

Dr. Cécile Lahellec (cecile.lahellec@club-internet.fr) is a honorary research director of the French Food Safety Agency (AFSSA), in Alfort (France). She is a veterinarian (Alfort, 1964) and food microbiologist (Pasteur Institute of Lille and Caen University, France; trainings in the United Kingdom and the USA). Her research was mostly devoted to poultry product microbiology for 27 years in Ploufragan (France), where she introduced the concept “from farm to fork” applied to pathogens such as Salmonella. Head of the Food hygiene central Laboratory (1991) and of the Department of Food hygiene, quality and safety (1992), both depending on the National Centre for Food and Veterinary Studies (CNEVA), in Paris (France). Director of Food Hygiene (1999) of the AFSSA, in which the CNEVA has been integrated. She early realizes the interest of miniaturized and other alternative methods, in which she becames much involved. Convenor (from 1993 to 2005) of the European Committee for Standardization (CEN) TC 275 working group no. 6. She has been involved in many French and international commissions.

 

Dr. Armand Sánchez Bonastre (armand.sanchez@uab.cat), head of the Molecular genetics veterinary Service of UAB, is a professor in the Departament de Ciència animal i dels aliments at UAB. Head of the research group “Molecular animal breeding in veterinary sciences”, whose activities focus on the application of molecular techniques to animal production. He is in charge of many projects financed by Spanish or international institutions, or private companies. At present, he takes part in animal genomics projects about genetic characterization of characteristics interesting for animal production, and in the development of analytical methods for molecular diagnosis. He has published more than 100 papers in international journals on molecular genetics applied to livestock production.

 

Mr. Martín Iorlano (miorlano@apsalab.com) is, from 2006, the head of the Microbiology Laboratory of APSALAB, SL, laboratory of analyses of medicine premixes, feed and raw materials for animal feeding, in Reus, Tarragona. He received his B.S. degree in Microbiology from Universidad Nacional de Río Cuarto (Argentina), and his master degree in Analysis and control from the Institut de Ciència i Tecnologia (IUCT). He plans and supervises the tasks in the laboratory, and also the validations and revalidations of methods. At present, the company is in the course of accreditation according to the ISO standard 17025 for food and feed microbiological assays.

 

Dr. Teresa Esteve Nuez (tengmp@cid.csic.es) is the head of the Quantitative biological analyses Service, for genetically modified organisms (GMOs) detection, of the Centre de Recerca en Agrigenòmica (CRAG), which belongs to the CSIC-IRTA-UAB consortium (Consejo Superior de Investigaciones Científicas-Institut de Recerca i Tecnologia Agroalimentàries-Universitat Autònoma de Barcelona), in Barcelona. Her speciality is molecular genetics. Most of her career has developed at several research areas of the CSIC, besides de current one: head of the Aminoacid analysis Service of the Centre d’Investigació i Desenvolupament (CID); DNA sequentiation Service of the Institut de Biologia Molecular de Barcelona (IBMB); and Departament d’Investigacions antropològiques i genètiques. Head of a research group that participates in several Spanish and European projects of the VI framework programme. Author of many publications about GMOs, including papers in scientific journals, book chapters and presentations in congresses.

 

Mr. David Tomás Fornés (dtomas@ainia.es) is, from 1999, the head of the Microbiology and molecular biology Laboratory of ainia.centro tecnológico, in Paterna, Valencia. He received his B.S. degree in Food science and technology from Universidad Politécnica de Valencia. He is the Spanish coordinator of the Committee for the Standardization of microbiological methods for foods of the Asociación Española de Normalización y Certificación (AENOR), and represents the Spanish group in the European Committee for Standardization (CEN) and the International Organization for Standardization (ISO). He is involved in the working group on development of ISO 16140 about the protocol for the validation of alternative microbiological methods. Deputy chairman of the Sociedad Española de Microbiología (SEM) Comisión de Normalización y Validación (CNV). He has taken part, as a CEN expert, in international cooperation projects about quality systems at official microbiology laboratories in the member countries of the Association of Southeast Asian Nations (ASEAN), and in other cooperation projects in Egypt. He has been in charge of projects financed by public institutions or private companies, about preparation and validation of analytical techniques.

 

Dr. Ferran Ribas Soler (feribsol@hotmail.com) was the head of the Microbiology Area of the Societat General d’Aigües de Barcelona (AGBAR) Laboratory, coordinating the biological aspects, including analyses and advisings to laboratories and industries, and quality management. As a microbiologist in AGBAR, he worked for forty years (from 1967 to 2007) in physicochemical and microbiological analyses, and bioassays in the water cycle. Member of the US Standard methods committee; member of the SEM board of directors; president of the SEM Comisión de Normalización y Validación; and Spain representative (AENOR expert) in working groups for ISO standards on water microbiology. He has participated in several projects, and the BDOC-BOM European-US and AGHTM-Biofilms European working groups. Author of 50 papers published in scientific journals, and more than 100 presentations in congresses, mainly about water pathogenic specific bacterial groups, viruses and protozoa; water contamination bacterial and viral indicators; biofilms; and equivalence among microbiological methods.

 

Dr. José Juan Rodríguez Jerez (josejuan.rodriguez@uab.cat), head of the Food safety Observatory of UAB, is an associate professor in the Departament de Ciència animal i dels aliments at UAB. He coordinates a research group, specialized in development of rapid methods for foodborne disease prevention. From 1996, he works on techniques based on impedance, immunology (ELISA and ELFA), ATP analysis, microscopy (light and fluorescence microscopy, and image analysis) and molecular biology (real-time PCR). At present, there are ten scientists on his research group, who have published almost 100 papers, 60 of which are research papers, and book chapters. From 2000, they are in charge of the scientific coordination of the website www.consumaseguridad.com.

 

Ms. Montse Vila Brugalla (mvila.brugalla@gmail.com) works at the Barcelona Public Health Agency. She was the head, for twelve years, of the Quality control Department of Central de Cocinados CATAR, SA, an industry that manufactures pasteurized ready-to-eat meals. She received her B.S. degree in Veterinary Science from UAB. Her interest in thermobacteriology and the way of establishing food shelf-life leads her to the knowledge of microbial growth and inactivation models, and predictive microbiology. In 2002, she defends her research work entitled “Study on the application of Gompertz equation and Baranyi model for establishment of pasteurized sauce shelf-life”, in the Food nutrition, technology and hygiene postgraduate programme. Since then, she attends courses and conferences related to that subject, where she focuses on the practical application.
 

Dr. Mª Isabel Pividori Gurgo (isabel.pividori@uab.cat) is an associate professor  in the Departament de Química at UAB, and has large experience in design of DNA electrochemical biosensors, which was the topic of her Ph.D. studies, the first ones on that field in Spain. She is doing research in the Grup de Sensors i Biosensors of the Departament de Química, which consists of 26 scientists and has published more than 400 papers, taking part in Spanish and international projects about biosensor (of DNA and immunosensors) design and development for food control, new methods for DNA and immunospecies rapid analyses, and analytical bioinstrumentation, based on composite bionanomaterials and magnetic particles. Director of the postgraduate course “Bioanalytical chemistry”, given in the Facultad de Bioquímica y ciencias biológicas at Universidad Nacional del Litoral (Argentina). Recipient of the 2005 Excellence National Award (Argentina), and the VIII European Associació de Químics de l’Institut Químic de Sarrià Award on Enzyme Technology.

 

 

MONOGRAPHS
 

Compilation of the lectures of past workshops, published as monographs in Alimentaria journal, official publication of workshop MRAMA.

 

VII workshop MRAMA

 

VI workshop MRAMA

 

V workshop MRAMA  

 

 

DOCUMENTS FROM MICROBIOLOGY COMPANIES

 

3M España, SA

·         Quality solutions for testing times

·         Enrichments and dilutions

·         Petrifilm

·         Petrifilm for aerobic bacteria

·         Petrifilm for Enterobacteriaceae

·         Petrifilm for E. coli and coliforms

·         Petrifilm for yeast and mould

·         Petrifilm Staph express

·         Petrifilm for environmental Listeria

·         Petrifilm for lactic acid bacteria

·         Petrifilm for lactic acid bacteria (guide)

·         Petrifilm: environmental monitoring procedures

·         Electronic Pipettor II

·         Clean-Trace

·         Tecra pathogen and toxin testing

·         Tecra Salmonella visual immunoassay

·         Tecra Staphylococcus aureus visual immunoassay

·         Tecra staphylococcal enterotoxins visual immunoassay

·         Tecra Bacillus diarrhoeal enterotoxin visual immunoassay

 

 

AES CHEMUNEX España, SA

·         Lab automation

·         ChemScan RDI, D-Count, BactiFlow

·         Real-time and standardised TVC analysis of raw meat and meat products

·         Dairy and fermented milk products ultra-rapid microbial testing

·         Soft drinks, and fruit juices, concentrates and preparations ultra-rapid microbial testing

·         CHEMUNEX technology to control product quality (Coca-Cola)

·         Sensitive analyses in a minute cycle (Eckes-granini)

·         Rapid testing reduces the production cycle

·         BiOLOG

·         BiOLOG: GEN III microplate

 

Applied Biosystems

·         7300, 7500, 7500 Fast, 7900, Step One y Step One Plus

·         MicroSEQ Salmonella spp. detection kit

·         MicroSEQ Listeria monocytogenes detection kit

·         PrepSEQ rapid spin sample preparation kit

 

BC Aplicaciones Analíticas, SL

·         Promilite III

·         systemSURE Plus + PRO-Clean, SpotCheck, others

 

BD Diagnostic Systems

·         BBL Campy-cefex agar and BBL CHROMagar

·         BBL Campy-cefex agar

·         BBL CHROMagar and other BD chromogenic culture media

·         BD identification systems

·         BBL Crystal ID (microbial taxa)

 

 

bioMérieux España, SA

·         Microbiological solutions for agri-food industries (catalogue)

·         air IDEAL

·         CampyFood ID agar and chromID agar

·         TEMPO

·         TEMPO EB (for Enterobacteriaceae) (technical data)

·         VITEK 2 compact

·         BacT/ALERT 3D

·         VIDAS

·         VIDAS next day

·         VIDAS Easy SML

·         VIDAS SMLX (protocol)

·         VIDAS LMX (protocol)

·         Recombinant phage protein

·         VIDAS UP E. coli O157:H7

·         VIDAS UP E. coli O157:H7 (technical data)

·         DiversiLab

 

Bio-Rad Laboratories, SA

·         RAPID’ chromogenic methods (i)

·         RAPID’ chromogenic methods (ii)

·         RAPID’E.coli 2

·         RAPID’E.coli O157:H7 (poster)

·         RAPID’Staph

·         RAPID’Listeria spp. (poster)

·         RAPID’L.mono (poster)

·         iQ-Check

·         iQ-Check E. coli O157:H7

·         iQ-Check Salmonella II

·         iQ-Check Listeria monocytogenes II

·         iQ-Check Listeria spp.

·         Listeria Special Broth (LSB) (poster)

·         iQ-Check Listeria spp. (poster)

·         Real-time PCR: equipment and iQ-Check kits (multimedia)

 

 

Bioser, SA

·         Pulsifier

·         MicroBio

·         Biokar Diagnostics chromogenic culture media

·         COMPASS Salmonella agar

·         Microgen ID

·         Path-Check Hygiene pathogen system and Clean Card Pro

·         Histamine analysis

·         Mycotoxin analysis

·         Gluten analysis

·         Biokits ELISA (allergens)

·         Microgen ID and Microgen latex

·         Microgen latex

·         RapidChek

·         RapidChek SELECT for Salmonella

·         RapidChek for Listeria

·         Biokits RAPID 3D (allergens)

·         Ovation BioNatural pipettes

·         Real-time PCR kits

·         Newsletter Microbial

·         Airfree

 

Biótica, SL

·         Immunomagnetic kit Bioalarm/Legionella

·         Immunomagnetic kit for rapid determination of Legionella: encouraging a microbiology socially responsible (multimedia)

 

Eppendorf

·         Mastercycler ep realplex

·         Mastercycler

·         twin.tec real-time PCR plates

·         epMotion

·         Mastercycler ep realplex (references)

·         Mastercycler ep realplex (application notes)

 
Fluka Analytical (Sigma-Aldrich Química, SA)

·         Clostridium perfringens ChromoSelect agar

·         HybriScan (i)

·         HybriScan (ii)

·         HybriScan D total bacterial count (protocol)

·         HybriScan: rapid test systems for microbial food diagnostics (multimedia)

 

 

GeneSystems, SA (part of Pall Life Sciences)

·         GeneDisc

 

 

IDEXX Laboratorios, SL

·         Colilert-18

·         Enterolert

·         Quanti-Tray and Quanti-Tray/2000

·         Alternative methods and their equivalence tests for microbiological control of drinking waters and recreational waters (multimedia)

 

IUL, SA

·         Spin Air

·         Smart Dilutor

·         Masticator

·         Spiral plating

·         Eddy Jet

·         Bio Dilutor

·         Flash & Go

·         Manual Colony Counter and Haloes-Caliper

·         Plate Handler

·         Poly Stainer

 

IZASA, SA

·         Critical factors for successful real-time PCR

·         Automated DNA extraction: QIAcube

 

LABAQUA, SA

·         Microbiological reference material (i)

·         Microbiological reference material (ii)

·         Practical applications of reference materials at microbiology laboratory (multimedia)

 

 

Laboratorios MICROKIT, SL

·         Dehydrated chromogenic culture media

·         Compact Dry Plate

·         Drink water kit P/A

·         KIT PRO-5S and KIT PRO-WATER for surface hygiene control

·         ListeriaKIT screening swabs for surface HACCP control

 

 

MATRIX MicroScience, Inc

·         PATHATRIX

·         PATHATRIX pooling strategy

·         PATHATRIX 10 pooling Salmonella assay

·         PATHATRIX (bibliography)

 

Micrology Laboratories, LLC

·         The Coliscan membrane filter method for E. coli and total coliforms (protocol)

·         The Coliscan membrane filter method for E. coli and total coliforms (instructions)

·         Easygel swab kit

 

 

MicroPlanet Laboratorios, SL: BioControl Systems, Inc

·         SimPlate

·         SimPlate for Campylobacter (guide)

·         LIGHTNING MVP

·         Transia Plate Total Aflatoxins

·         Transia Plate Aflatoxin B1

·         Transia Plate Aflatoxin M1

·         Transia Plate DON (deoxynivalenol)

·         Transia Plate Fumonisins

·         Transia Plate Zearalenone

·         b-Lactoglobulin Residue ELISA

·         Casein Residue ELISA

·         Egg Residue ELISA

·         Crustacean Tropomyosin Residue ELISA

·         Almond Residue ELISA

·         Hazelnut Residue ELISA

·         Peanut Residue ELISA

·         Soy Protein Residue ELISA

·         Mustard Seed Residue ELISA

·         Sesame Seed Residue ELISA

·         VIP

·         VIP for Listeria (guide)

·         VIP Gold

·         VIP Gold for Salmonella (guide)

·         VIP Gold for EHEC (guide)

·         VIP Gold for Listeria (guide)

·         1-2 Test for Salmonella

·         1-2 Test for Salmonella (guide)

·         1-2 Test for Salmonella (instructions)

 

MicroPlanet Laboratorios, SL: LIOFILCHEM, srl

·         Contact slide

·         Chromatic DETECTION

·         EnteroPluri-Test

·         FOOD-SYSTEM

·         Clean Test

 

 

Nirco, SL

·         SAS

·         Dilucup/Dilushaker

·         E. sakazakii medium

·         NEO-GRID (i)

·         NEO-GRID (ii)

·         Whitley jar gassing system

·         AccuPoint

·         Soleris

·         Veratox for histamine

·         Reveal for pathogenic bacteria

·         Reveal for peanut allergen

·         Reveal for total milk allergen

·         Mycotoxins

·         GeneQuence

·         Rapid tests for microbiological screening (multimedia)

 

 

Olympus España, SAU

·         AmpliGrid/AmpliSpeed system

·         AmpliGrid/AmpliSpeed system (specifications)

·         AmpliGrid/AmpliSpeed system (video)

·         AmpliGrid AG480F/AmpliSpeed slide cycler ASC200D (application report )

 

Oxoid, SA (parte de Thermo Fisher Scientific, Inc)

·         Oxoid Brilliance E. coli/coliform agar

·         Oxoid Salmonella Precis method

·         Oxoid Salmonella Precis method (poster)

·         Oxoid Listeria Precis method

·         Listeria: rapid tests, AFNOR-validated method

·         O·B·I·S·

·         O·B·I·S· Campy

·         BAX Q7

·         BAX Q7 Listeria

·         RiboPrinter

 
Roche Diagnostics, SL

·         LightCycler (i)

·         LightCycler (ii)

 

 

LANGUAGES

 

English and Spanish.

Simultaneous translation will be provided.

 

 

FEES

 

The registration fee for the laboratory sessions is 50 €.

 

Rest of the workshop:

·         UAB students: 12 € (plus 15 € if handout material is required)

·         UAB staff: 40 €

·         Non-UAB students: 125 € (or 65 €/1 day)

·         ACCA members: 175 €

·         Alimentaria; Alimentación, Equipos y Tecnología; or Técnicas de Laboratorio journal subscribers: 190 €

·         Others: 215 € (or 110 €/1 day)

 

Students will have to prove their position.

 

 

PAYMENT

 

By bank transfer.

The name of the bank is la Caixa, and the number of the account is:

IBAN-ES10-2100-0424-3702-0019-3047

BIC/CODIGO SWIFT CAIXESBBXXX.

Include “VIII workshop MRAMA”, and your first and last names.

Send the receipt by e-mail or fax.

 

 

ACCOMMODATION

 

 

QUESTIONS

 

If you have questions concerning the workshop, contact:

 

Josep Yuste (josep.yuste@uab.cat)

Marta Capellas (marta.capellas@uab.cat)

' +34-93-5811446 / 6 +34-93-5811494

 

 

Registration form

 

Last name: ..........................................................

First name: ..........................................................

ID card: ...............................................................

Occupation: ........................................................

Company: ...........................................................

Address: ..............................................................

Postcode: ............................................................

Town and country:...............................................

Telephone: ..........................................................

Fax: .....................................................................

E-mail: ................................................................

 

Days of attendance to the workshop:

r Tuesday 24th

r Wednesday 25th

r Thursday 26th

 

Laboratory sessions (only one option):

r mornings (Wednesday 25th, Thursday 26th and Friday 27th)

 

Activities on Friday 27th:

·        Demonstration on Use of internet for applications of predictive microbiology:

r 11:15 a.m.-12:15 p.m.

 

·        Demonstration on Electrochemical immunosensors for pathogen detection:    

r 12:15-1 p.m.

 

VAT number and company address (if an invoice is required):

 

 

 

Send this form by e-mail or fax.

 

 

GALLERY OF PICTURES

 

VIII workshop MRAMA:

·        Official picture

·        Others:

See pictures Tuesday 24

Download pictures Tuesday 24

See pictures Wednesday 25

Download pictures Wednesday 25

See pictures Thursday 26

Download pictures Thursday 26

See pictures Friday 27

Download pictures Friday 27

 

VII workshop MRAMA: Official picture

 

VI workshop MRAMA:

·        Official picture

·        Others:

- Picture 1

- Picture 2

- Picture 3

 

V workshop MRAMA: Official picture

 

IV workshop MRAMA: Official picture

 

III workshop MRAMA: Official picture

 

II workshop MRAMA: Official picture

 

I workshop MRAMA: Picture

 

 

Great microbiologists (and other SCIENTISTS) of the history

 

·        Antonie Philips van Leeuwenhoek (Delft, the Netherlands, 1632 – 1723)   

·        Lazzaro Spallanzani (Scandiano, Italy, 1729 – Pavia, Italy, 1799)

·        Christian Gottfried Ehrenberg (Delitzsch, Germany, 1795 – Berlin, Germany, 1876)

·        John Tyndall (Leighlinbridge, Ireland, 1820 – Hindhead, United Kingdom, 1893)

·        Louis Pasteur (Dole, France, 1822 – Saint-Cloud, France, 1895)

·        Ferdinand Julius Cohn (Wroclaw, Poland, 1828 – 1898)

·        Heinrich Hermann Robert Koch (Clausthal-Zellerfeld, Germany, 1843 – Baden-Baden, Germany, 1910)

·   &nb