VIII
WORKSHOP
RAPID METHODS AND
AUTOMATION
IN FOOD
MICROBIOLOGY
(
Universitat
Autònoma de Barcelona
Lecture
hall
(with
sessions in laboratory)
Bellaterra
(Cerdanyola del Vallès),
24th
to 27th November 2009
Departament de Ciència animal i dels aliments
![]() |
PURPOSE
To broaden and spread the
theoretical and practical knowledges about innovative methods for rapid
detection, count, isolation and characterization of foodborne and waterborne
microorganisms.
KEYWORDS
Rapid microbiology ·
Automation · Miniaturization · Food safety · Pathogens · Food analysis
DESIGNED FOR
Directors
and technicians of laboratories, consultancies and industries of food, and
other sectors (clinical, pharmaceutical, cosmetic, chemical, environmental, and
so on); food inspectors and other government staff; people from microbiology
companies; university teachers, technical staff, and graduate and postgraduate students; people from other research centres; and so on.
Tuesday, 24th November
9:30-10:15 a.m. Opening lecture
by Dr. Cécile Lahellec (Agence
Française de Sécurité Sanitaire des Aliments
–AFSSA–, Alfort, France):
·
“International cooperation in food microbiology”
10:15-11:15 a.m. Dr. Daniel Y. C. Fung:
·
Sampling and sample preparation of solid and liquid foods, surfaces, and air
·
Miniaturization:
microtiter plate, most probable number (MPN), viable cell counts, and so on.
Diagnostic kits
11:15-11:45 a.m. Break
11:45 a.m.-12:45 p.m. Dr. D. Y. C. Fung:
·
Methods
for viable cell counts: hydrophobic grid, spiral plating, flow cytometry,
direct epifluorescent filter technique (DEFT), and so on
·
Other
methods to detect microorganisms: Fung’s double tube, Lee tube, Fung-Yu tube,
and so on.
12:45-2:15 p.m. Lunch
2:15-3 p.m. Dr. D. Y. C. Fung:
·
Methods
for viable cell counts, based on electric impedance, conductance and
capacitance
·
Methods
for viable cell counts, based on bioluminiscence (ATP analysis) and colorimetry
3-4 p.m. Dr.
Armand Sánchez Bonastre (Universitat Autònoma de Barcelona –UAB–,
Bellaterra –Cerdanyola del Vallès,
·
“La polymerase chain reaction (PCR)” [“Polymerase
chain reaction (PCR)”]
4-4:30 p.m. Sr. Martín Iorlano (APSALAB, SL, Reus,
Tarragona):
·
“Puesta a punto e implantación de
4:30-4:40 p.m. Break
4:40-6:50 p.m. Microbiology companies: 2 exhibitions:![]()
* The
industrial traditional microbiology: first dilution; homogenization; spiral
dilution; colony counting; and database communication among all steps (IUL, SA)
* BAX
Q7 system: simple and consistent PCR, designed for the needs of food industry,
and reference and official control laboratories (Oxoid, SA –part of Thermo Fisher Scientific, Inc–)
+![]()
* BD DS: systems
and products for water and food microbiological analyses, and environmental
control (Becton Dickinson, SA)![]()
* Application of
real-time PCR to routine detection of food pathogens (GeneSystems, SA –part of Pall Life Sciences–)
* Development and validation of Brucella spp. molecular typification assays by
conventional PCR and real-time PCR (Roche
Diagnostics, SL)![]()
6:50 p.m. Merienda
Wednesday, 25th November
8:30-
FULL!
11-11:15 a.m. Break
11:15 a.m.-1 p.m. Dr. D. Y. C. Fung:
·
Immunological methods for microbial and toxin identification:
immunomagnetic separation, ELISA and ELFA, reversed passive latex
agglutination, lateral immunomigration, immunoprecipitation, and so on
1-2:15 p.m. Lunch
2:15-3 p.m. Dr. Teresa Esteve Nuez (Centre de Recerca en Agrigenòmica –CRAG–, CSIC-IRTA-UAB consortium,
·
“Organismos
modificados
genéticamente (OMGs): detección, legislación y evaluación de riesgos” [“Genetically
modified organisms (GMOs): detection, legislation and risk assessment”]
3-3:45 p.m. Mr. David Tomás Fornés (ainia.centro tecnológico, Paterna, Valencia):
·
“Control de calidad interno en laboratorios de microbiología” [“Internal quality control in microbiology laboratories”]
3:45-4 p.m. Break
4-6:30 p.m. Microbiology companies: 4
exhibitions:![]()
* Microbiology laboratory automation and
traceability. Rapid microbiology solutions (AES
CHEMUNEX España, SA)
* Microbiology laboratory automation for
detection and counting: a fact (bioMérieux España, SA)![]()
* ATP – Bioluminiscence and concentration of
disinfectants for HACCP audits. Immunomagnetic
separation for foodborne
pathogen detection (MicroPlanet
Laboratorios, SL: BioControl Systems,
Inc / LIOFILCHEM, srl)
+![]()
* AmpliGrid technology
for PCR, real-time PCR and sequentiation. Also from one single cell! (
6:30 p.m. Merienda
Thursday, 26th November
8:30-
FULL!
11-11:15 a.m. Break
11:15-
·
Genetic
methods for microbial identification: hybridization (DNA and RNAr
probes); polymerase chain reaction (PCR); DNA characterization
(fingerprinting, riboprinting); biosensors, biochips and microchips;
proteomics; and so on
·
“Estudios de equivalencia europeos entre métodos para enumerar Escherichia
coli y enterococos en aguas de baño” [“European equivalence studies between methods
for Escherichia coli and enterococci enumeration in recreational waters”]
12:45-1 p.m. Official picture
1-2:15 p.m. Lunch
2:15-3:15 p.m. Open discussion about
instrumentation in food microbiology, world trade trends, and other current
topics. Conclusions. Participants:
-
Chairman: Dr. José Juan Rodríguez Jerez (Universitat
Autònoma de Barcelona)
-
Dr. Daniel. Y. C. Fung
-
Dr. Cécile Lahellec
-
Dr. Ferran Ribas Soler
-
Mr. Xavier Lizana Alcazo (ACONSA –Asesoría y Consultoría Sanitaria, SL–)
-
Mr. Francesc García Bejarano (bioMérieux España, SA)
-
Mr. Juan Antonio García González (Applied Biosystems)
3:15-4 p.m. Official adjournment
of the workshop: attendance-certificates-giving and celebration
4-6:30 p.m. Microbiology
companies: 4 exhibitions:
* Rapid methods related to quality management in
the food industry (3M
España, SA)![]()
* Implementation of foodborne pathogen detection
systems at quality control laboratories (Applied Biosystems)
* Optimization at quality laboratory:
pathogen analysis and surface hygiene validation (Bioser, SA)![]()
* Eppendorf:
a complete system for real-time PCR (Eppendorf)![]()
Friday, 27th November
9-
FULL!
11:15
a.m.-12:15 p.m. Demonstration on Use of internet for applications of predictive microbiology (limited attendance),
by Ms. Montse Vila Brugalla (Barcelona
Public Health Agency) FULL!
12:15-1 p.m. Demonstration
on Electrochemical immunosensors for
pathogen detection (limited attendance), by Drs. Mª Isabel Pividori
Gurgo and Anabel Lermo Soria, and Ms. Susana Liébana Girona (UAB) FULL!
In these sessions, which
will be given by Drs.
Mª Manuela Hernández Herrero (manuela.hernandez@uab.cat)
and Artur Xavier Roig
Sagués
(arturxavier.roig@uab.cat),
different rapid methods will be assayed: Surface
and air samplers: contact
plates,
Rodac plates, contact slides, Sampl’air, MicroBio. Homogenizer
Pulsifier; blender Smasher. Dilumat S and Dilubag. Dilucup/Dilushaker.
Chromogenic
culture media: ASAP, SMS, ESIA, REBECCA, ALOA, BACARA,
Baird-Parker RPF agar, chromID Coli, chromID Salmonella, chromID O157:H7,
chromID Ottaviani Agosti, Brilliance
Salmonella agar (Oxoid Salmonella Precis method), Brilliance Listeria agar (Oxoid Listeria Precis method), Rose-Gal BCIG agar, SESAME
Salmonella test, COMPASS Salmonella
agar, COMPASS Listeria agar, CONFIRM’
L. mono agar, COMPASS Bacillus cereus agar, BBL CHROMagar
Salmonella, BBL CHROMagar O157, BBL CHROMagar Listeria, BBL CHROMagar Staph
aureus, Chromatic DETECTION Salmonella,
Compact
Dry Plate ETB, Compact Dry Plate SL, Compact Dry Plate X-SA. Rotating plater Twister. Spiral plater WASP II. Colony counter EC2.
Petrifilm (plates and reader). NEO-GRID. Easygel. SimPlate. TEMPO (miniaturized and automated MPN). Diagnostic kits and
readers: API, BBL Enterotube II, BBL Crystal ID, RapID ONE,
O·B·I·S·, Microbact, Microgen ID, EnteroPluri-Test, FOOD-SYSTEM. ATP – Bioluminiscence: luminometers Luminomat, Clean-Trace NG, LIGHTNING MVP. Colorimetry:
Clean Test, drink water kit P/A, KIT PRO. Immunology: ELFA/ELISA (VIDAS UP E. coli O157:H7: phage recombinant
protein technology, Alert for histamine), reversed passive latex agglutination (Microgen latex, Oxoid latex test), lateral immunomigration (RapidChek, BioKits
RAPID 3D –allergens–, Reveal –pathogens, allergens–, VIP),
immunoprecipitation (1-2 Test for Salmonella).
Others. Explanation about
hands-free "Pop-up" adhesive tape sampling, Easygel, Fung’s double
tube and Fung-Yu tube.
Attendance will be limited to 25 people per session.
MICROBIOLOGy
companies: EXHIBItion
People from companies will explain and show how
their equipments and products work: Samplers: air IDEAL, Spin Air, Spin Air Slave, Spin Air
Basic, Basic Air. Pinch
dilutor. Preparator of culture media, and automated pourer stacker APS One. Plate
Handler. Homogenizers: Masticator Silver
Panoramic, Masticator Compact Panoramic; blender Smasher. Dilumat S and Dilubag. Dilutors:
Smart Dilutor, Bio Dilutor. Chromogenic culture media: SESAME Salmonella
test, COMPASS Listeria agar, CONFIRM’
L. mono agar. Spiral plater Eddy Jet. Colony counters and
inhibition halo readers: Manual Colony Counter, Haloes-Caliper, Flash & Go.
Petrifilm. Staining automation: Poly Stainer. Real-time microbiology CHEMUNEX: BactiFlow.
TEMPO (miniaturized and automated MPN). BiOLOG identification system. eviSENSE monitoring
system. ATP – Bioluminiscence: luminometer LIGHTNING MVP. Colorimetry:
Path-Check Hygiene. Immunology: ELFA (VIDAS), lateral
immunomigration (BioKits RAPID 3D –allergens– + multichannel Ovation pipette),
immunomagnetic separation PickPen. Molecular biology: PCR (7300, 7500, 7500 Fast, 7900, Step One, Step One
Plus; BAX Q7; ADIAFOOD; LightCycler; AmpliGrid; Mastercycler ep realplex; Assurance GDS; GeneDisc; kits Salmofast, Listerfast, Legiofast, Campylofast,
Tyrofast, Sakafast). AirFree. Others.
Dr. Daniel Y. C. Fung (dfung@ksu.edu) is a professor of food science in the
Department of Animal sciences and industry at Kansas State University (KSU), in
Dr. Cécile Lahellec (cecile.lahellec@club-internet.fr)
is a honorary research director of the French Food Safety Agency (AFSSA), in
Alfort (France). She is
a veterinarian (Alfort, 1964) and food microbiologist
(Pasteur Institute of
Dr. Armand Sánchez
Bonastre (armand.sanchez@uab.cat), head of the Molecular
genetics veterinary Service of UAB, is a professor in the Departament de Ciència animal i dels aliments at UAB. Head of the
research group “Molecular animal breeding in veterinary sciences”, whose
activities focus on the application of molecular techniques to animal
production. He is in charge of many projects financed by Spanish or
international institutions, or private companies. At present, he takes part in
animal genomics projects about genetic characterization of characteristics
interesting for animal production, and in the development of analytical methods
for molecular diagnosis. He has published more than 100 papers in international
journals on molecular genetics applied to livestock production.
Mr. Martín Iorlano (miorlano@apsalab.com) is, from 2006, the head of the Microbiology
Laboratory of APSALAB, SL, laboratory of analyses of
medicine premixes, feed and raw materials for animal feeding, in
Dr. Teresa Esteve Nuez
(tengmp@cid.csic.es) is the head of the Quantitative biological analyses Service, for
genetically modified organisms (GMOs) detection, of the Centre de Recerca en Agrigenòmica (CRAG), which belongs to the
CSIC-IRTA-UAB consortium (Consejo
Superior de Investigaciones Científicas-Institut
de Recerca i Tecnologia Agroalimentàries-Universitat Autònoma de Barcelona), in Barcelona. Her speciality is
molecular genetics. Most of her career has developed at several research areas
of the CSIC, besides de current one: head of the Aminoacid analysis Service of
the Centre d’Investigació i
Desenvolupament (CID); DNA sequentiation Service of the Institut de Biologia Molecular de Barcelona (IBMB);
and Departament d’Investigacions
antropològiques i genètiques. Head of a research group that participates in
several Spanish and European projects of the VI framework programme. Author of
many publications about GMOs, including papers in scientific journals, book
chapters and presentations in congresses.
Mr. David Tomás Fornés (dtomas@ainia.es) is, from 1999, the head of the Microbiology
and molecular biology Laboratory of ainia.centro tecnológico, in
Dr. Ferran Ribas Soler (feribsol@hotmail.com) was the
head of the Microbiology Area of the Societat General d’Aigües de Barcelona
(AGBAR) Laboratory, coordinating the biological aspects,
including analyses and advisings to laboratories and industries, and quality
management. As a microbiologist in AGBAR, he worked for forty years (from 1967
to 2007) in physicochemical and microbiological analyses, and bioassays
in the water cycle. Member of the
Dr. José Juan
Rodríguez Jerez (josejuan.rodriguez@uab.cat), head
of the Food safety Observatory of UAB, is an associate professor in the Departament
de Ciència animal i dels aliments at UAB. He coordinates a research group,
specialized in development of rapid methods for foodborne disease prevention.
From 1996, he works on techniques based on impedance, immunology (ELISA and
ELFA), ATP analysis, microscopy (light and fluorescence microscopy, and image
analysis) and molecular biology (real-time PCR). At present, there are ten
scientists on his research group, who have published almost 100 papers, 60 of
which are research papers, and book chapters. From 2000, they are in charge of the
scientific coordination of the website www.consumaseguridad.com.
Dr.
Mª Isabel Pividori Gurgo (isabel.pividori@uab.cat) is an associate professor in the Departament de
Química at UAB, and has large experience in design of DNA electrochemical
biosensors, which was the topic of her Ph.D. studies, the first ones on that
field in
Compilation
of the lectures of past workshops, published as monographs in Alimentaria journal, official publication of workshop MRAMA.
|
|
BC
Aplicaciones Analíticas, SL
|
|
|
·
Microbiological
solutions for agri-food industries (catalogue) |
|
|
·
VIDAS UP E.
coli O157:H7 (technical data) |
|
|
|
·
Immunomagnetic kit for
rapid determination of Legionella: encouraging
a microbiology socially responsible (multimedia) |
|
·
HybriScan: rapid test systems for microbial food diagnostics (multimedia) |
|
GeneSystems, SA (part of Pall Life Sciences)
|
|
|
·
Alternative methods and their equivalence tests for
microbiological control of drinking waters and recreational waters
(multimedia) |
|
·
Practical applications of reference materials at
microbiology laboratory (multimedia) |
|
|
|
|
·
The Coliscan
membrane filter method for E. coli
and total coliforms (protocol) |
·
The Coliscan
membrane filter method for E. coli
and total coliforms (instructions) |
|
|
MicroPlanet Laboratorios, SL: BioControl Systems, Inc
MicroPlanet Laboratorios, SL: LIOFILCHEM, srl
|
|
|
|
|
·
AmpliGrid AG480F/AmpliSpeed slide
cycler ASC200D
(application report ) |
Oxoid, SA (parte de Thermo Fisher Scientific, Inc)
LANGUAGES
English and Spanish.
Simultaneous translation
will be provided.
FEES
The registration fee for the laboratory sessions is 50 €.
Rest of the workshop:
·
UAB
students: 12 € (plus 15 € if handout material is required)
·
UAB
staff: 40 €
·
Non-UAB
students: 125 € (or 65 €/1 day)
·
ACCA
members: 175 €
·
Alimentaria; Alimentación, Equipos y Tecnología; or Técnicas de Laboratorio journal subscribers: 190 €
·
Others:
215 € (or 110 €/1 day)
Students will have to prove their position.
By bank transfer.
The name of the bank is ”
IBAN-ES10-2100-0424-3702-0019-3047
BIC/CODIGO SWIFT CAIXESBBXXX.
Include “VIII workshop MRAMA”, and your first
and last names.
Send the receipt by e-mail
or fax.
QUESTIONS
If you have questions concerning the workshop,
contact:
Josep Yuste (josep.yuste@uab.cat)
Marta Capellas (marta.capellas@uab.cat)
' +34-93-5811446 / 6 +34-93-5811494
Registration form
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ID card:
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Address: ..............................................................
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Days of attendance to the workshop:
r Tuesday 24th
r Wednesday 25th
r Thursday 26th
Laboratory sessions (only one
option):
r mornings (Wednesday 25th, Thursday 26th
and Friday 27th)
Activities on Friday
27th:
·
Demonstration on Use
of internet for applications of predictive microbiology:
r 11:15 a.m.-12:15
p.m.
·
Demonstration on Electrochemical
immunosensors for pathogen detection:
r 12:15-1 p.m.
VAT number
and company address (if an invoice is required):
Send
this form by e-mail or fax.
VIII
workshop MRAMA:
· Others:
· Others:
·
Antonie Philips van Leeuwenhoek (
·
Lazzaro Spallanzani (Scandiano, Italy, 1729 – Pavia, Italy, 1799)
·
Christian Gottfried Ehrenberg (Delitzsch, Germany, 1795 – Berlin,
Germany, 1876)![]()
·
John Tyndall (Leighlinbridge, Ireland, 1820 – Hindhead,
United Kingdom, 1893)![]()
·
Louis Pasteur (Dole, France, 1822 – Saint-Cloud, France, 1895)
·
Ferdinand Julius Cohn (Wroclaw, Poland, 1828 – 1898)![]()
·
Heinrich Hermann Robert Koch (Clausthal-Zellerfeld, Germany,
1843 – Baden-Baden, Germany, 1910)
· &nb